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p0753s commercial assay  (New England Biolabs)


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    New England Biolabs p0753s commercial assay
    P0753s Commercial Assay, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 2909 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p0753s+commercial+assay/Lambda+Protein+Phosphatase/10__7554_slash_elife__74277-253-70-66
    Average 99 stars, based on 2909 article reviews
    p0753s commercial assay - by Bioz Stars, 2026-09
    99/100 stars

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    Recombinant:

    Article Title: A bidirectional switch in the Shank3 phosphorylation state biases synapses toward up- or downscaling
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Rattus norvegicus) Long–Evans Charles River Laboratories Strain: 006; RRID:RGD_2308852 Strain, strain background (Mus musculus) C57BL/6 Charles River Laboratories Strain:027; RRID:IMSR_ CRL:27 Wu, Tatavarty, Jean Beltran, et al. eLife 2022;11:e74277.

    Article Title: IRAK1-dependent Regnase-1-14-3-3 complex formation controls Regnase-1-mediated mRNA decay
    Article Snippet: DOI: https://doi.org/10.7554/eLife.71966 15 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Recombinant DNA reagent pMD2.G Addgene RRID:Addgene_12259 Recombinant DNA reagent pMDLg/pRRE Addgene RRID:Addgene_12251 Recombinant DNA reagent pRSV-Rev Addgene RRID:Addgene_12253 Recombinant DNA reagent pInducer20 Addgene RRID:Addgene_44012 Recombinant DNA reagent pInducer20-puro this paper NeoR of pInducer20 (Addgene_44012) was replaced with PuroR Recombinant DNA reagent pFLAG-CMV2 Sigma Cat # E7033 Recombinant DNA reagent pEGFP-C1 Clontech Peptide, recombinant protein FLAG Peptide Sigma Cat # F3290 Peptide, recombinant protein HA peptide MBL Life science Cat # 3320 HA tagged Protein PURIFICATION KIT Peptide, recombinant protein recombinant human IL-1b R and D Systems Cat # 201-LB-005 Peptide, recombinant protein recombinant mouse IL-1b BioLegend Cat # 575102 Peptide, recombinant protein recombinant human IL-17A BioLegend Cat # 570502 Peptide, recombinant protein recombinant human TNF BioLegend Cat # 570104 Commercial assay or kit Dynabeads Protein G Invitrogen Cat # 10004D Commercial assay or kit Lambda Protein Phosphatase NEB Cat # P0753S Commercial assay or kit Signal Enhancer HIKARI nacalai tesque Cat # 02270-81 Commercial assay or kit Immobilon Forte Western HRP Substrate Millipore Cat # WBLUF0500 Commercial assay or kit TRIzol Reagent Invitrogen Cat # 15596018 Commercial assay or kit RNA Clean and Concentrator-5 Zymo Research Cat # R1014 Commercial assay or kit PowerUp SYBR Green Master Mix Applied Biosystems Cat # A25742 Commercial assay or kit IL-6 Mouse Uncoated ELISA Kit Invitrogen Cat # 88-7064-88 Chemical compound, drug DSP (dithiobis(succinimidyl propionate)) TCI Cat # D2473 Chemical compound, drug Pam3CSK4 InvivoGen Cat # tlrl-pms Chemical compound, drug poly I:C cytiva Cat # 27473201 Chemical compound, drug LPS InvivoGen Cat # tlrl-smlps Chemical compound, drug R848 InvivoGen Cat # tlrl-r848-5 Chemical compound, drug CpG DNA InvivoGen Cat # tlrl-1668-1 ODN 1668 Chemical compound, drug MG-132 Sigma Cat # 474790 Chemical compound, drug Actinomycin D Sigma Cat # A9415 Chemical compound, drug Leptomycin B Sigma Cat # L2913

    Bicinchoninic Acid Protein Assay:

    Article Title: A bidirectional switch in the Shank3 phosphorylation state biases synapses toward up- or downscaling
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Rattus norvegicus) Long–Evans Charles River Laboratories Strain: 006; RRID:RGD_2308852 Strain, strain background (Mus musculus) C57BL/6 Charles River Laboratories Strain:027; RRID:IMSR_ CRL:27 Wu, Tatavarty, Jean Beltran, et al. eLife 2022;11:e74277.

    Article Title: IRAK1-dependent Regnase-1-14-3-3 complex formation controls Regnase-1-mediated mRNA decay
    Article Snippet: DOI: https://doi.org/10.7554/eLife.71966 15 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Recombinant DNA reagent pMD2.G Addgene RRID:Addgene_12259 Recombinant DNA reagent pMDLg/pRRE Addgene RRID:Addgene_12251 Recombinant DNA reagent pRSV-Rev Addgene RRID:Addgene_12253 Recombinant DNA reagent pInducer20 Addgene RRID:Addgene_44012 Recombinant DNA reagent pInducer20-puro this paper NeoR of pInducer20 (Addgene_44012) was replaced with PuroR Recombinant DNA reagent pFLAG-CMV2 Sigma Cat # E7033 Recombinant DNA reagent pEGFP-C1 Clontech Peptide, recombinant protein FLAG Peptide Sigma Cat # F3290 Peptide, recombinant protein HA peptide MBL Life science Cat # 3320 HA tagged Protein PURIFICATION KIT Peptide, recombinant protein recombinant human IL-1b R and D Systems Cat # 201-LB-005 Peptide, recombinant protein recombinant mouse IL-1b BioLegend Cat # 575102 Peptide, recombinant protein recombinant human IL-17A BioLegend Cat # 570502 Peptide, recombinant protein recombinant human TNF BioLegend Cat # 570104 Commercial assay or kit Dynabeads Protein G Invitrogen Cat # 10004D Commercial assay or kit Lambda Protein Phosphatase NEB Cat # P0753S Commercial assay or kit Signal Enhancer HIKARI nacalai tesque Cat # 02270-81 Commercial assay or kit Immobilon Forte Western HRP Substrate Millipore Cat # WBLUF0500 Commercial assay or kit TRIzol Reagent Invitrogen Cat # 15596018 Commercial assay or kit RNA Clean and Concentrator-5 Zymo Research Cat # R1014 Commercial assay or kit PowerUp SYBR Green Master Mix Applied Biosystems Cat # A25742 Commercial assay or kit IL-6 Mouse Uncoated ELISA Kit Invitrogen Cat # 88-7064-88 Chemical compound, drug DSP (dithiobis(succinimidyl propionate)) TCI Cat # D2473 Chemical compound, drug Pam3CSK4 InvivoGen Cat # tlrl-pms Chemical compound, drug poly I:C cytiva Cat # 27473201 Chemical compound, drug LPS InvivoGen Cat # tlrl-smlps Chemical compound, drug R848 InvivoGen Cat # tlrl-r848-5 Chemical compound, drug CpG DNA InvivoGen Cat # tlrl-1668-1 ODN 1668 Chemical compound, drug MG-132 Sigma Cat # 474790 Chemical compound, drug Actinomycin D Sigma Cat # A9415 Chemical compound, drug Leptomycin B Sigma Cat # L2913

    Magnetic Beads:

    Article Title: A bidirectional switch in the Shank3 phosphorylation state biases synapses toward up- or downscaling
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Rattus norvegicus) Long–Evans Charles River Laboratories Strain: 006; RRID:RGD_2308852 Strain, strain background (Mus musculus) C57BL/6 Charles River Laboratories Strain:027; RRID:IMSR_ CRL:27 Wu, Tatavarty, Jean Beltran, et al. eLife 2022;11:e74277.

    Article Title: IRAK1-dependent Regnase-1-14-3-3 complex formation controls Regnase-1-mediated mRNA decay
    Article Snippet: DOI: https://doi.org/10.7554/eLife.71966 15 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Recombinant DNA reagent pMD2.G Addgene RRID:Addgene_12259 Recombinant DNA reagent pMDLg/pRRE Addgene RRID:Addgene_12251 Recombinant DNA reagent pRSV-Rev Addgene RRID:Addgene_12253 Recombinant DNA reagent pInducer20 Addgene RRID:Addgene_44012 Recombinant DNA reagent pInducer20-puro this paper NeoR of pInducer20 (Addgene_44012) was replaced with PuroR Recombinant DNA reagent pFLAG-CMV2 Sigma Cat # E7033 Recombinant DNA reagent pEGFP-C1 Clontech Peptide, recombinant protein FLAG Peptide Sigma Cat # F3290 Peptide, recombinant protein HA peptide MBL Life science Cat # 3320 HA tagged Protein PURIFICATION KIT Peptide, recombinant protein recombinant human IL-1b R and D Systems Cat # 201-LB-005 Peptide, recombinant protein recombinant mouse IL-1b BioLegend Cat # 575102 Peptide, recombinant protein recombinant human IL-17A BioLegend Cat # 570502 Peptide, recombinant protein recombinant human TNF BioLegend Cat # 570104 Commercial assay or kit Dynabeads Protein G Invitrogen Cat # 10004D Commercial assay or kit Lambda Protein Phosphatase NEB Cat # P0753S Commercial assay or kit Signal Enhancer HIKARI nacalai tesque Cat # 02270-81 Commercial assay or kit Immobilon Forte Western HRP Substrate Millipore Cat # WBLUF0500 Commercial assay or kit TRIzol Reagent Invitrogen Cat # 15596018 Commercial assay or kit RNA Clean and Concentrator-5 Zymo Research Cat # R1014 Commercial assay or kit PowerUp SYBR Green Master Mix Applied Biosystems Cat # A25742 Commercial assay or kit IL-6 Mouse Uncoated ELISA Kit Invitrogen Cat # 88-7064-88 Chemical compound, drug DSP (dithiobis(succinimidyl propionate)) TCI Cat # D2473 Chemical compound, drug Pam3CSK4 InvivoGen Cat # tlrl-pms Chemical compound, drug poly I:C cytiva Cat # 27473201 Chemical compound, drug LPS InvivoGen Cat # tlrl-smlps Chemical compound, drug R848 InvivoGen Cat # tlrl-r848-5 Chemical compound, drug CpG DNA InvivoGen Cat # tlrl-1668-1 ODN 1668 Chemical compound, drug MG-132 Sigma Cat # 474790 Chemical compound, drug Actinomycin D Sigma Cat # A9415 Chemical compound, drug Leptomycin B Sigma Cat # L2913

    IP Phosphatase Assay:

    Article Title: A bidirectional switch in the Shank3 phosphorylation state biases synapses toward up- or downscaling
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Rattus norvegicus) Long–Evans Charles River Laboratories Strain: 006; RRID:RGD_2308852 Strain, strain background (Mus musculus) C57BL/6 Charles River Laboratories Strain:027; RRID:IMSR_ CRL:27 Wu, Tatavarty, Jean Beltran, et al. eLife 2022;11:e74277.

    Article Title: IRAK1-dependent Regnase-1-14-3-3 complex formation controls Regnase-1-mediated mRNA decay
    Article Snippet: DOI: https://doi.org/10.7554/eLife.71966 15 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Recombinant DNA reagent pMD2.G Addgene RRID:Addgene_12259 Recombinant DNA reagent pMDLg/pRRE Addgene RRID:Addgene_12251 Recombinant DNA reagent pRSV-Rev Addgene RRID:Addgene_12253 Recombinant DNA reagent pInducer20 Addgene RRID:Addgene_44012 Recombinant DNA reagent pInducer20-puro this paper NeoR of pInducer20 (Addgene_44012) was replaced with PuroR Recombinant DNA reagent pFLAG-CMV2 Sigma Cat # E7033 Recombinant DNA reagent pEGFP-C1 Clontech Peptide, recombinant protein FLAG Peptide Sigma Cat # F3290 Peptide, recombinant protein HA peptide MBL Life science Cat # 3320 HA tagged Protein PURIFICATION KIT Peptide, recombinant protein recombinant human IL-1b R and D Systems Cat # 201-LB-005 Peptide, recombinant protein recombinant mouse IL-1b BioLegend Cat # 575102 Peptide, recombinant protein recombinant human IL-17A BioLegend Cat # 570502 Peptide, recombinant protein recombinant human TNF BioLegend Cat # 570104 Commercial assay or kit Dynabeads Protein G Invitrogen Cat # 10004D Commercial assay or kit Lambda Protein Phosphatase NEB Cat # P0753S Commercial assay or kit Signal Enhancer HIKARI nacalai tesque Cat # 02270-81 Commercial assay or kit Immobilon Forte Western HRP Substrate Millipore Cat # WBLUF0500 Commercial assay or kit TRIzol Reagent Invitrogen Cat # 15596018 Commercial assay or kit RNA Clean and Concentrator-5 Zymo Research Cat # R1014 Commercial assay or kit PowerUp SYBR Green Master Mix Applied Biosystems Cat # A25742 Commercial assay or kit IL-6 Mouse Uncoated ELISA Kit Invitrogen Cat # 88-7064-88 Chemical compound, drug DSP (dithiobis(succinimidyl propionate)) TCI Cat # D2473 Chemical compound, drug Pam3CSK4 InvivoGen Cat # tlrl-pms Chemical compound, drug poly I:C cytiva Cat # 27473201 Chemical compound, drug LPS InvivoGen Cat # tlrl-smlps Chemical compound, drug R848 InvivoGen Cat # tlrl-r848-5 Chemical compound, drug CpG DNA InvivoGen Cat # tlrl-1668-1 ODN 1668 Chemical compound, drug MG-132 Sigma Cat # 474790 Chemical compound, drug Actinomycin D Sigma Cat # A9415 Chemical compound, drug Leptomycin B Sigma Cat # L2913

    Sequencing:

    Article Title: A bidirectional switch in the Shank3 phosphorylation state biases synapses toward up- or downscaling
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Rattus norvegicus) Long–Evans Charles River Laboratories Strain: 006; RRID:RGD_2308852 Strain, strain background (Mus musculus) C57BL/6 Charles River Laboratories Strain:027; RRID:IMSR_ CRL:27 Wu, Tatavarty, Jean Beltran, et al. eLife 2022;11:e74277.

    Article Title: IRAK1-dependent Regnase-1-14-3-3 complex formation controls Regnase-1-mediated mRNA decay
    Article Snippet: DOI: https://doi.org/10.7554/eLife.71966 15 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Recombinant DNA reagent pMD2.G Addgene RRID:Addgene_12259 Recombinant DNA reagent pMDLg/pRRE Addgene RRID:Addgene_12251 Recombinant DNA reagent pRSV-Rev Addgene RRID:Addgene_12253 Recombinant DNA reagent pInducer20 Addgene RRID:Addgene_44012 Recombinant DNA reagent pInducer20-puro this paper NeoR of pInducer20 (Addgene_44012) was replaced with PuroR Recombinant DNA reagent pFLAG-CMV2 Sigma Cat # E7033 Recombinant DNA reagent pEGFP-C1 Clontech Peptide, recombinant protein FLAG Peptide Sigma Cat # F3290 Peptide, recombinant protein HA peptide MBL Life science Cat # 3320 HA tagged Protein PURIFICATION KIT Peptide, recombinant protein recombinant human IL-1b R and D Systems Cat # 201-LB-005 Peptide, recombinant protein recombinant mouse IL-1b BioLegend Cat # 575102 Peptide, recombinant protein recombinant human IL-17A BioLegend Cat # 570502 Peptide, recombinant protein recombinant human TNF BioLegend Cat # 570104 Commercial assay or kit Dynabeads Protein G Invitrogen Cat # 10004D Commercial assay or kit Lambda Protein Phosphatase NEB Cat # P0753S Commercial assay or kit Signal Enhancer HIKARI nacalai tesque Cat # 02270-81 Commercial assay or kit Immobilon Forte Western HRP Substrate Millipore Cat # WBLUF0500 Commercial assay or kit TRIzol Reagent Invitrogen Cat # 15596018 Commercial assay or kit RNA Clean and Concentrator-5 Zymo Research Cat # R1014 Commercial assay or kit PowerUp SYBR Green Master Mix Applied Biosystems Cat # A25742 Commercial assay or kit IL-6 Mouse Uncoated ELISA Kit Invitrogen Cat # 88-7064-88 Chemical compound, drug DSP (dithiobis(succinimidyl propionate)) TCI Cat # D2473 Chemical compound, drug Pam3CSK4 InvivoGen Cat # tlrl-pms Chemical compound, drug poly I:C cytiva Cat # 27473201 Chemical compound, drug LPS InvivoGen Cat # tlrl-smlps Chemical compound, drug R848 InvivoGen Cat # tlrl-r848-5 Chemical compound, drug CpG DNA InvivoGen Cat # tlrl-1668-1 ODN 1668 Chemical compound, drug MG-132 Sigma Cat # 474790 Chemical compound, drug Actinomycin D Sigma Cat # A9415 Chemical compound, drug Leptomycin B Sigma Cat # L2913

    Software:

    Article Title: A bidirectional switch in the Shank3 phosphorylation state biases synapses toward up- or downscaling
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Rattus norvegicus) Long–Evans Charles River Laboratories Strain: 006; RRID:RGD_2308852 Strain, strain background (Mus musculus) C57BL/6 Charles River Laboratories Strain:027; RRID:IMSR_ CRL:27 Wu, Tatavarty, Jean Beltran, et al. eLife 2022;11:e74277.

    Article Title: IRAK1-dependent Regnase-1-14-3-3 complex formation controls Regnase-1-mediated mRNA decay
    Article Snippet: DOI: https://doi.org/10.7554/eLife.71966 15 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Recombinant DNA reagent pMD2.G Addgene RRID:Addgene_12259 Recombinant DNA reagent pMDLg/pRRE Addgene RRID:Addgene_12251 Recombinant DNA reagent pRSV-Rev Addgene RRID:Addgene_12253 Recombinant DNA reagent pInducer20 Addgene RRID:Addgene_44012 Recombinant DNA reagent pInducer20-puro this paper NeoR of pInducer20 (Addgene_44012) was replaced with PuroR Recombinant DNA reagent pFLAG-CMV2 Sigma Cat # E7033 Recombinant DNA reagent pEGFP-C1 Clontech Peptide, recombinant protein FLAG Peptide Sigma Cat # F3290 Peptide, recombinant protein HA peptide MBL Life science Cat # 3320 HA tagged Protein PURIFICATION KIT Peptide, recombinant protein recombinant human IL-1b R and D Systems Cat # 201-LB-005 Peptide, recombinant protein recombinant mouse IL-1b BioLegend Cat # 575102 Peptide, recombinant protein recombinant human IL-17A BioLegend Cat # 570502 Peptide, recombinant protein recombinant human TNF BioLegend Cat # 570104 Commercial assay or kit Dynabeads Protein G Invitrogen Cat # 10004D Commercial assay or kit Lambda Protein Phosphatase NEB Cat # P0753S Commercial assay or kit Signal Enhancer HIKARI nacalai tesque Cat # 02270-81 Commercial assay or kit Immobilon Forte Western HRP Substrate Millipore Cat # WBLUF0500 Commercial assay or kit TRIzol Reagent Invitrogen Cat # 15596018 Commercial assay or kit RNA Clean and Concentrator-5 Zymo Research Cat # R1014 Commercial assay or kit PowerUp SYBR Green Master Mix Applied Biosystems Cat # A25742 Commercial assay or kit IL-6 Mouse Uncoated ELISA Kit Invitrogen Cat # 88-7064-88 Chemical compound, drug DSP (dithiobis(succinimidyl propionate)) TCI Cat # D2473 Chemical compound, drug Pam3CSK4 InvivoGen Cat # tlrl-pms Chemical compound, drug poly I:C cytiva Cat # 27473201 Chemical compound, drug LPS InvivoGen Cat # tlrl-smlps Chemical compound, drug R848 InvivoGen Cat # tlrl-r848-5 Chemical compound, drug CpG DNA InvivoGen Cat # tlrl-1668-1 ODN 1668 Chemical compound, drug MG-132 Sigma Cat # 474790 Chemical compound, drug Actinomycin D Sigma Cat # A9415 Chemical compound, drug Leptomycin B Sigma Cat # L2913

    Protein Purification:

    Article Title: A bidirectional switch in the Shank3 phosphorylation state biases synapses toward up- or downscaling
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Rattus norvegicus) Long–Evans Charles River Laboratories Strain: 006; RRID:RGD_2308852 Strain, strain background (Mus musculus) C57BL/6 Charles River Laboratories Strain:027; RRID:IMSR_ CRL:27 Wu, Tatavarty, Jean Beltran, et al. eLife 2022;11:e74277.

    Article Title: IRAK1-dependent Regnase-1-14-3-3 complex formation controls Regnase-1-mediated mRNA decay
    Article Snippet: DOI: https://doi.org/10.7554/eLife.71966 15 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Recombinant DNA reagent pMD2.G Addgene RRID:Addgene_12259 Recombinant DNA reagent pMDLg/pRRE Addgene RRID:Addgene_12251 Recombinant DNA reagent pRSV-Rev Addgene RRID:Addgene_12253 Recombinant DNA reagent pInducer20 Addgene RRID:Addgene_44012 Recombinant DNA reagent pInducer20-puro this paper NeoR of pInducer20 (Addgene_44012) was replaced with PuroR Recombinant DNA reagent pFLAG-CMV2 Sigma Cat # E7033 Recombinant DNA reagent pEGFP-C1 Clontech Peptide, recombinant protein FLAG Peptide Sigma Cat # F3290 Peptide, recombinant protein HA peptide MBL Life science Cat # 3320 HA tagged Protein PURIFICATION KIT Peptide, recombinant protein recombinant human IL-1b R and D Systems Cat # 201-LB-005 Peptide, recombinant protein recombinant mouse IL-1b BioLegend Cat # 575102 Peptide, recombinant protein recombinant human IL-17A BioLegend Cat # 570502 Peptide, recombinant protein recombinant human TNF BioLegend Cat # 570104 Commercial assay or kit Dynabeads Protein G Invitrogen Cat # 10004D Commercial assay or kit Lambda Protein Phosphatase NEB Cat # P0753S Commercial assay or kit Signal Enhancer HIKARI nacalai tesque Cat # 02270-81 Commercial assay or kit Immobilon Forte Western HRP Substrate Millipore Cat # WBLUF0500 Commercial assay or kit TRIzol Reagent Invitrogen Cat # 15596018 Commercial assay or kit RNA Clean and Concentrator-5 Zymo Research Cat # R1014 Commercial assay or kit PowerUp SYBR Green Master Mix Applied Biosystems Cat # A25742 Commercial assay or kit IL-6 Mouse Uncoated ELISA Kit Invitrogen Cat # 88-7064-88 Chemical compound, drug DSP (dithiobis(succinimidyl propionate)) TCI Cat # D2473 Chemical compound, drug Pam3CSK4 InvivoGen Cat # tlrl-pms Chemical compound, drug poly I:C cytiva Cat # 27473201 Chemical compound, drug LPS InvivoGen Cat # tlrl-smlps Chemical compound, drug R848 InvivoGen Cat # tlrl-r848-5 Chemical compound, drug CpG DNA InvivoGen Cat # tlrl-1668-1 ODN 1668 Chemical compound, drug MG-132 Sigma Cat # 474790 Chemical compound, drug Actinomycin D Sigma Cat # A9415 Chemical compound, drug Leptomycin B Sigma Cat # L2913

    Western Blot:

    Article Title: A bidirectional switch in the Shank3 phosphorylation state biases synapses toward up- or downscaling
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Rattus norvegicus) Long–Evans Charles River Laboratories Strain: 006; RRID:RGD_2308852 Strain, strain background (Mus musculus) C57BL/6 Charles River Laboratories Strain:027; RRID:IMSR_ CRL:27 Wu, Tatavarty, Jean Beltran, et al. eLife 2022;11:e74277.

    Article Title: IRAK1-dependent Regnase-1-14-3-3 complex formation controls Regnase-1-mediated mRNA decay
    Article Snippet: DOI: https://doi.org/10.7554/eLife.71966 15 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Recombinant DNA reagent pMD2.G Addgene RRID:Addgene_12259 Recombinant DNA reagent pMDLg/pRRE Addgene RRID:Addgene_12251 Recombinant DNA reagent pRSV-Rev Addgene RRID:Addgene_12253 Recombinant DNA reagent pInducer20 Addgene RRID:Addgene_44012 Recombinant DNA reagent pInducer20-puro this paper NeoR of pInducer20 (Addgene_44012) was replaced with PuroR Recombinant DNA reagent pFLAG-CMV2 Sigma Cat # E7033 Recombinant DNA reagent pEGFP-C1 Clontech Peptide, recombinant protein FLAG Peptide Sigma Cat # F3290 Peptide, recombinant protein HA peptide MBL Life science Cat # 3320 HA tagged Protein PURIFICATION KIT Peptide, recombinant protein recombinant human IL-1b R and D Systems Cat # 201-LB-005 Peptide, recombinant protein recombinant mouse IL-1b BioLegend Cat # 575102 Peptide, recombinant protein recombinant human IL-17A BioLegend Cat # 570502 Peptide, recombinant protein recombinant human TNF BioLegend Cat # 570104 Commercial assay or kit Dynabeads Protein G Invitrogen Cat # 10004D Commercial assay or kit Lambda Protein Phosphatase NEB Cat # P0753S Commercial assay or kit Signal Enhancer HIKARI nacalai tesque Cat # 02270-81 Commercial assay or kit Immobilon Forte Western HRP Substrate Millipore Cat # WBLUF0500 Commercial assay or kit TRIzol Reagent Invitrogen Cat # 15596018 Commercial assay or kit RNA Clean and Concentrator-5 Zymo Research Cat # R1014 Commercial assay or kit PowerUp SYBR Green Master Mix Applied Biosystems Cat # A25742 Commercial assay or kit IL-6 Mouse Uncoated ELISA Kit Invitrogen Cat # 88-7064-88 Chemical compound, drug DSP (dithiobis(succinimidyl propionate)) TCI Cat # D2473 Chemical compound, drug Pam3CSK4 InvivoGen Cat # tlrl-pms Chemical compound, drug poly I:C cytiva Cat # 27473201 Chemical compound, drug LPS InvivoGen Cat # tlrl-smlps Chemical compound, drug R848 InvivoGen Cat # tlrl-r848-5 Chemical compound, drug CpG DNA InvivoGen Cat # tlrl-1668-1 ODN 1668 Chemical compound, drug MG-132 Sigma Cat # 474790 Chemical compound, drug Actinomycin D Sigma Cat # A9415 Chemical compound, drug Leptomycin B Sigma Cat # L2913

    SYBR Green Assay:

    Article Title: A bidirectional switch in the Shank3 phosphorylation state biases synapses toward up- or downscaling
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Rattus norvegicus) Long–Evans Charles River Laboratories Strain: 006; RRID:RGD_2308852 Strain, strain background (Mus musculus) C57BL/6 Charles River Laboratories Strain:027; RRID:IMSR_ CRL:27 Wu, Tatavarty, Jean Beltran, et al. eLife 2022;11:e74277.

    Article Title: IRAK1-dependent Regnase-1-14-3-3 complex formation controls Regnase-1-mediated mRNA decay
    Article Snippet: DOI: https://doi.org/10.7554/eLife.71966 15 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Recombinant DNA reagent pMD2.G Addgene RRID:Addgene_12259 Recombinant DNA reagent pMDLg/pRRE Addgene RRID:Addgene_12251 Recombinant DNA reagent pRSV-Rev Addgene RRID:Addgene_12253 Recombinant DNA reagent pInducer20 Addgene RRID:Addgene_44012 Recombinant DNA reagent pInducer20-puro this paper NeoR of pInducer20 (Addgene_44012) was replaced with PuroR Recombinant DNA reagent pFLAG-CMV2 Sigma Cat # E7033 Recombinant DNA reagent pEGFP-C1 Clontech Peptide, recombinant protein FLAG Peptide Sigma Cat # F3290 Peptide, recombinant protein HA peptide MBL Life science Cat # 3320 HA tagged Protein PURIFICATION KIT Peptide, recombinant protein recombinant human IL-1b R and D Systems Cat # 201-LB-005 Peptide, recombinant protein recombinant mouse IL-1b BioLegend Cat # 575102 Peptide, recombinant protein recombinant human IL-17A BioLegend Cat # 570502 Peptide, recombinant protein recombinant human TNF BioLegend Cat # 570104 Commercial assay or kit Dynabeads Protein G Invitrogen Cat # 10004D Commercial assay or kit Lambda Protein Phosphatase NEB Cat # P0753S Commercial assay or kit Signal Enhancer HIKARI nacalai tesque Cat # 02270-81 Commercial assay or kit Immobilon Forte Western HRP Substrate Millipore Cat # WBLUF0500 Commercial assay or kit TRIzol Reagent Invitrogen Cat # 15596018 Commercial assay or kit RNA Clean and Concentrator-5 Zymo Research Cat # R1014 Commercial assay or kit PowerUp SYBR Green Master Mix Applied Biosystems Cat # A25742 Commercial assay or kit IL-6 Mouse Uncoated ELISA Kit Invitrogen Cat # 88-7064-88 Chemical compound, drug DSP (dithiobis(succinimidyl propionate)) TCI Cat # D2473 Chemical compound, drug Pam3CSK4 InvivoGen Cat # tlrl-pms Chemical compound, drug poly I:C cytiva Cat # 27473201 Chemical compound, drug LPS InvivoGen Cat # tlrl-smlps Chemical compound, drug R848 InvivoGen Cat # tlrl-r848-5 Chemical compound, drug CpG DNA InvivoGen Cat # tlrl-1668-1 ODN 1668 Chemical compound, drug MG-132 Sigma Cat # 474790 Chemical compound, drug Actinomycin D Sigma Cat # A9415 Chemical compound, drug Leptomycin B Sigma Cat # L2913

    Enzyme-linked Immunosorbent Assay:

    Article Title: A bidirectional switch in the Shank3 phosphorylation state biases synapses toward up- or downscaling
    Article Snippet: Reagent type (species) or resource Designation Source or reference Identifiers Additional information Strain, strain background (Rattus norvegicus) Long–Evans Charles River Laboratories Strain: 006; RRID:RGD_2308852 Strain, strain background (Mus musculus) C57BL/6 Charles River Laboratories Strain:027; RRID:IMSR_ CRL:27 Wu, Tatavarty, Jean Beltran, et al. eLife 2022;11:e74277.

    Article Title: IRAK1-dependent Regnase-1-14-3-3 complex formation controls Regnase-1-mediated mRNA decay
    Article Snippet: DOI: https://doi.org/10.7554/eLife.71966 15 of 29 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Recombinant DNA reagent pMD2.G Addgene RRID:Addgene_12259 Recombinant DNA reagent pMDLg/pRRE Addgene RRID:Addgene_12251 Recombinant DNA reagent pRSV-Rev Addgene RRID:Addgene_12253 Recombinant DNA reagent pInducer20 Addgene RRID:Addgene_44012 Recombinant DNA reagent pInducer20-puro this paper NeoR of pInducer20 (Addgene_44012) was replaced with PuroR Recombinant DNA reagent pFLAG-CMV2 Sigma Cat # E7033 Recombinant DNA reagent pEGFP-C1 Clontech Peptide, recombinant protein FLAG Peptide Sigma Cat # F3290 Peptide, recombinant protein HA peptide MBL Life science Cat # 3320 HA tagged Protein PURIFICATION KIT Peptide, recombinant protein recombinant human IL-1b R and D Systems Cat # 201-LB-005 Peptide, recombinant protein recombinant mouse IL-1b BioLegend Cat # 575102 Peptide, recombinant protein recombinant human IL-17A BioLegend Cat # 570502 Peptide, recombinant protein recombinant human TNF BioLegend Cat # 570104 Commercial assay or kit Dynabeads Protein G Invitrogen Cat # 10004D Commercial assay or kit Lambda Protein Phosphatase NEB Cat # P0753S Commercial assay or kit Signal Enhancer HIKARI nacalai tesque Cat # 02270-81 Commercial assay or kit Immobilon Forte Western HRP Substrate Millipore Cat # WBLUF0500 Commercial assay or kit TRIzol Reagent Invitrogen Cat # 15596018 Commercial assay or kit RNA Clean and Concentrator-5 Zymo Research Cat # R1014 Commercial assay or kit PowerUp SYBR Green Master Mix Applied Biosystems Cat # A25742 Commercial assay or kit IL-6 Mouse Uncoated ELISA Kit Invitrogen Cat # 88-7064-88 Chemical compound, drug DSP (dithiobis(succinimidyl propionate)) TCI Cat # D2473 Chemical compound, drug Pam3CSK4 InvivoGen Cat # tlrl-pms Chemical compound, drug poly I:C cytiva Cat # 27473201 Chemical compound, drug LPS InvivoGen Cat # tlrl-smlps Chemical compound, drug R848 InvivoGen Cat # tlrl-r848-5 Chemical compound, drug CpG DNA InvivoGen Cat # tlrl-1668-1 ODN 1668 Chemical compound, drug MG-132 Sigma Cat # 474790 Chemical compound, drug Actinomycin D Sigma Cat # A9415 Chemical compound, drug Leptomycin B Sigma Cat # L2913



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